6OB5

Computationally-designed, modular sense/response system (S3-2D)

6OB5 の概要

• エントリーDOI: 10.2210/pdb6ob5/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900001
• 分子名称: Maltodextrin-binding protein, Ankyrin Repeat Domain (AR), S3-2D variant, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: computational protein design, chemically-induced dimerization, biosensor, rosetta, sugar binding protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 118275.27

構造登録者

Thompson, M.C.,Glasgow, A.A.,Huang, Y.M.,Fraser, J.S.,Kortemme, T. (登録日: 2019-03-19, 公開日: 2019-12-04, 最終更新日: 2023-10-11)

主引用文献

Glasgow, A.A.,Huang, Y.M.,Mandell, D.J.,Thompson, M.,Ritterson, R.,Loshbaugh, A.L.,Pellegrino, J.,Krivacic, C.,Pache, R.A.,Barlow, K.A.,Ollikainen, N.,Jeon, D.,Kelly, M.J.S.,Fraser, J.S.,Kortemme, T.
Computational design of a modular protein sense-response system.
Science, 366:1024-1028, 2019

PubMed Abstract: Sensing and responding to signals is a fundamental ability of living systems, but despite substantial progress in the computational design of new protein structures, there is no general approach for engineering arbitrary new protein sensors. Here, we describe a generalizable computational strategy for designing sensor-actuator proteins by building binding sites de novo into heterodimeric protein-protein interfaces and coupling ligand sensing to modular actuation through split reporters. Using this approach, we designed protein sensors that respond to farnesyl pyrophosphate, a metabolic intermediate in the production of valuable compounds. The sensors are functional in vitro and in cells, and the crystal structure of the engineered binding site closely matches the design model. Our computational design strategy opens broad avenues to link biological outputs to new signals.

PubMed: 31754004
DOI: 10.1126/science.aax8780

実験手法

X-RAY DIFFRACTION (2.208 Å)