6O4M

Racemic melittin

6O4M の概要

• エントリーDOI: 10.2210/pdb6o4m/pdb
• 分子名称: D-Melittin, Melittin, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: racemic, cytotoxic, antimicrobial, toxin
• 由来する生物種: Apis mellifera (Honeybee); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 12260.51

構造登録者

Kurgan, K.W.,Bingman, C.A.,Gellman, S.H.,Forest, K.T. (登録日: 2019-02-28, 公開日: 2019-05-22, 最終更新日: 2024-11-06)

主引用文献

Kurgan, K.W.,Kleman, A.F.,Bingman, C.A.,Kreitler, D.F.,Weisblum, B.,Forest, K.T.,Gellman, S.H.
Retention of Native Quaternary Structure in Racemic Melittin Crystals.
J.Am.Chem.Soc., 141:7704-7708, 2019

PubMed Abstract: Racemic crystallography has been used to elucidate the secondary and tertiary structures of peptides and small proteins that are recalcitrant to conventional crystallization. It is unclear, however, whether racemic crystallography can capture native quaternary structure, which could be disrupted by heterochiral associations. We are exploring the use of racemic crystallography to characterize the self-assembly behavior of membrane-associated peptides, very few of which have been crystallized. We report a racemic crystal structure of the membrane-active peptide melittin; the new structure allows comparison with a previously reported crystal structure of L-melittin. The tetrameric assembly observed in crystalline L-melittin has been proposed to represent the tetrameric state detected in solution for this peptide. This tetrameric assembly is precisely reproduced in the racemic crystal, which strengthens the conclusion that the tetramer is biologically relevant. More broadly, these findings suggest that racemic crystallography can provide insight on native quaternary structure.

PubMed: 31059253
DOI: 10.1021/jacs.9b02691

実験手法

X-RAY DIFFRACTION (1.27 Å)