6O43
Crystal structure of a lysin protein from Staphylococcus phage P68
Summary for 6O43
| Entry DOI | 10.2210/pdb6o43/pdb |
| Descriptor | Orf11, 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid (3 entities in total) |
| Functional Keywords | lysin, bacteriophage protein, antimicrobial protein |
| Biological source | Staphylococcus phage P68 |
| Total number of polymer chains | 1 |
| Total formula weight | 22485.54 |
| Authors | Truong, J.Q. (deposition date: 2019-02-27, release date: 2019-07-10, Last modification date: 2024-03-13) |
| Primary citation | Truong, J.Q.,Panjikar, S.,Shearwin-Whyatt, L.,Bruning, J.B.,Shearwin, K.E. Combining random microseed matrix screening and the magic triangle for the efficient structure solution of a potential lysin from bacteriophage P68. Acta Crystallogr D Struct Biol, 75:670-681, 2019 Cited by PubMed Abstract: Two commonly encountered bottlenecks in the structure determination of a protein by X-ray crystallography are screening for conditions that give high-quality crystals and, in the case of novel structures, finding derivatization conditions for experimental phasing. In this study, the phasing molecule 5-amino-2,4,6-triiodoisophthalic acid (I3C) was added to a random microseed matrix screen to generate high-quality crystals derivatized with I3C in a single optimization experiment. I3C, often referred to as the magic triangle, contains an aromatic ring scaffold with three bound I atoms. This approach was applied to efficiently phase the structures of hen egg-white lysozyme and the N-terminal domain of the Orf11 protein from Staphylococcus phage P68 (Orf11 NTD) using SAD phasing. The structure of Orf11 NTD suggests that it may play a role as a virion-associated lysin or endolysin. PubMed: 31282476DOI: 10.1107/S2059798319009008 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.08222992502 Å) |
Structure validation
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