6O2H

Hen lysozyme in triclinic space group at ambient temperature - diffuse scattering dataset

6O2H の概要

• エントリーDOI: 10.2210/pdb6o2h/pdb
• 関連するPDBエントリー: 6O2G
• 分子名称: Lysozyme C, NITRATE ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: room temperature, diffuse scattering, lysozyme, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14800.65

構造登録者

Meisburger, S.P.,Ando, N. (登録日: 2019-02-22, 公開日: 2020-02-26, 最終更新日: 2024-10-23)

主引用文献

Meisburger, S.P.,Case, D.A.,Ando, N.
Diffuse X-ray scattering from correlated motions in a protein crystal.
Nat Commun, 11:1271-1271, 2020

PubMed Abstract: Protein dynamics are integral to biological function, yet few techniques are sensitive to collective atomic motions. A long-standing goal of X-ray crystallography has been to combine structural information from Bragg diffraction with dynamic information contained in the diffuse scattering background. However, the origin of macromolecular diffuse scattering has been poorly understood, limiting its applicability. We present a finely sampled diffuse scattering map from triclinic lysozyme with unprecedented accuracy and detail, clearly resolving both the inter- and intramolecular correlations. These correlations are studied theoretically using both all-atom molecular dynamics and simple vibrational models. Although lattice dynamics reproduce most of the diffuse pattern, protein internal dynamics, which include hinge-bending motions, are needed to explain the short-ranged correlations revealed by Patterson analysis. These insights lay the groundwork for animating crystal structures with biochemically relevant motions.

PubMed: 32152274
DOI: 10.1038/s41467-020-14933-6

実験手法

X-RAY DIFFRACTION (1.212 Å)