6O14

Crystal structure of the Aquifex aeolicus Wzt Carbohydrate Binding Domain

6O14 の概要

• エントリーDOI: 10.2210/pdb6o14/pdb
• 関連するPDBエントリー: 2R5O 5HNO 6AN7
• 分子名称: ABC transporter, GLYCEROL (3 entities in total)
• 機能のキーワード: abc transporter, wzt, wzm, o antigen, carbohydrate binding domain, membrane protein
• 由来する生物種: Aquifex aeolicus VF5
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35587.07

構造登録者

Bi, Y.,Zimmer, J. (登録日: 2019-02-17, 公開日: 2020-01-15, 最終更新日: 2023-10-11)

主引用文献

Bi, Y.,Zimmer, J.
Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain.
Structure, 28:252-, 2020

PubMed Abstract: A hallmark of Gram-negative bacteria is an asymmetric outer membrane containing lipopolysaccharides (LPSs) in the extracellular leaflet. LPS molecules consist of lipid A, which is connected to the inner and outer core oligosaccharides. This LPS core structure is extended in the periplasm by the O antigen, a variable and serotype-defining polysaccharide. In the ABC transporter-dependent LPS biosynthesis pathway, the WzmWzt transporter secretes the complete O antigen across the inner membrane for ligation to the LPS core. In some O antigen transporters, the nucleotide-binding domain of Wzt is fused C-terminally to a carbohydrate-binding domain (CBD) that interacts with the O antigen chain. Here, we present the crystal structure of the Aquifex aeolicus CBD that reveals a conserved flat and a variable twisted jelly-roll surface. The CBD dimer is stabilized by mutual β strand exchange. Microbial glycan array binding studies with the isolated CBD provide insights into its interaction with complex carbohydrates.

PubMed: 31879128
DOI: 10.1016/j.str.2019.11.020

実験手法

X-RAY DIFFRACTION (2.65 Å)