6O08

Gluconobacter Ene-Reductase (GluER)

6O08 の概要

• エントリーDOI: 10.2210/pdb6o08/pdb
• 分子名称: N-ethylmaleimide reductase, FLAVIN MONONUCLEOTIDE, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: g oxydans, old yellow enzyme, lactam cyclase, wt gluer, oxidoreductase
• 由来する生物種: Gluconobacter oxydans (Gluconobacter suboxydans)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41588.02

構造登録者

Garfinkle, S.E.,Jeffrey, P.,Hyster, T.K. (登録日: 2019-02-15, 公開日: 2019-06-26, 最終更新日: 2023-10-11)

主引用文献

Biegasiewicz, K.F.,Cooper, S.J.,Gao, X.,Oblinsky, D.G.,Kim, J.H.,Garfinkle, S.E.,Joyce, L.A.,Sandoval, B.A.,Scholes, G.D.,Hyster, T.K.
Photoexcitation of flavoenzymes enables a stereoselective radical cyclization.
Science, 364:1166-1169, 2019

PubMed Abstract: Photoexcitation is a common strategy for initiating radical reactions in chemical synthesis. We found that photoexcitation of flavin-dependent "ene"-reductases changes their catalytic function, enabling these enzymes to promote an asymmetric radical cyclization. This reactivity enables the construction of five-, six-, seven-, and eight-membered lactams with stereochemical preference conferred by the enzyme active site. After formation of a prochiral radical, the enzyme guides the delivery of a hydrogen atom from flavin-a challenging feat for small-molecule chemical reagents. The initial electron transfer occurs through direct excitation of an electron donor-acceptor complex that forms between the substrate and the reduced flavin cofactor within the enzyme active site. Photoexcitation of promiscuous flavoenzymes has thus furnished a previously unknown biocatalytic reaction.

PubMed: 31221855
DOI: 10.1126/science.aaw1143

実験手法

X-RAY DIFFRACTION (1.798 Å)