6NYC

Munc13-1 C2B-domain, calcium free

「3KWT」から置き換えられました

6NYC の概要

• エントリーDOI: 10.2210/pdb6nyc/pdb
• 分子名称: Munc13-1, CHLORIDE ION, 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• 機能のキーワード: phospholipid binding protein, metal binding protein
• 由来する生物種: Rattus norvegicus (Rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17290.37

構造登録者

Tomchick, D.R.,Rizo, J.,Machius, M.,Lu, J. (登録日: 2019-02-11, 公開日: 2019-02-20, 最終更新日: 2023-10-11)

主引用文献

Shin, O.H.,Lu, J.,Rhee, J.S.,Tomchick, D.R.,Pang, Z.P.,Wojcik, S.M.,Camacho-Perez, M.,Brose, N.,Machius, M.,Rizo, J.,Rosenmund, C.,Sudhof, T.C.
Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis.
Nat. Struct. Mol. Biol., 17:280-288, 2010

PubMed Abstract: Munc13 is a multidomain protein present in presynaptic active zones that mediates the priming and plasticity of synaptic vesicle exocytosis, but the mechanisms involved remain unclear. Here we use biophysical, biochemical and electrophysiological approaches to show that the central C(2)B domain of Munc13 functions as a Ca(2+) regulator of short-term synaptic plasticity. The crystal structure of the C(2)B domain revealed an unusual Ca(2+)-binding site with an amphipathic alpha-helix. This configuration confers onto the C(2)B domain unique Ca(2+)-dependent phospholipid-binding properties that favor phosphatidylinositolphosphates. A mutation that inactivated Ca(2+)-dependent phospholipid binding to the C(2)B domain did not alter neurotransmitter release evoked by isolated action potentials, but it did depress release evoked by action-potential trains. In contrast, a mutation that increased Ca(2+)-dependent phosphatidylinositolbisphosphate binding to the C(2)B domain enhanced release evoked by isolated action potentials and by action-potential trains. Our data suggest that, during repeated action potentials, Ca(2+) and phosphatidylinositolphosphate binding to the Munc13 C(2)B domain potentiate synaptic vesicle exocytosis, thereby offsetting synaptic depression induced by vesicle depletion.

PubMed: 20154707
DOI: 10.1038/nsmb.1758

実験手法

X-RAY DIFFRACTION (1.893 Å)