6NWC

PYL10 bound to the ABA pan-agonist 3CB

6NWC の概要

• エントリーDOI: 10.2210/pdb6nwc/pdb
• 分子名称: Abscisic acid receptor PYL10, 1-{[(4-cyano-3-cyclopropylphenyl)acetyl]amino}cyclohexane-1-carboxylic acid (3 entities in total)
• 機能のキーワード: pyr/pyl/rcar, pyl10, hormone receptor, plant protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17573.07

構造登録者

Peterson, F.C.,Vaidya, A.,Jensen, D.R.,Volkman, B.F.,Cutler, S.R. (登録日: 2019-02-06, 公開日: 2019-11-06, 最終更新日: 2023-10-11)

主引用文献

Vaidya, A.S.,Helander, J.D.M.,Peterson, F.C.,Elzinga, D.,Dejonghe, W.,Kaundal, A.,Park, S.Y.,Xing, Z.,Mega, R.,Takeuchi, J.,Khanderahoo, B.,Bishay, S.,Volkman, B.F.,Todoroki, Y.,Okamoto, M.,Cutler, S.R.
Dynamic control of plant water use using designed ABA receptor agonists.
Science, 366:-, 2019

PubMed Abstract: Drought causes crop losses worldwide, and its impact is expected to increase as the world warms. This has motivated the development of small-molecule tools for mitigating the effects of drought on agriculture. We show here that current leads are limited by poor bioactivity in wheat, a widely grown staple crop, and in tomato. To address this limitation, we combined virtual screening, x-ray crystallography, and structure-guided design to develop opabactin (OP), an abscisic acid (ABA) mimic with up to an approximately sevenfold increase in receptor affinity relative to ABA and up to 10-fold greater activity in vivo. Studies in reveal a role of the type III receptor for the antitranspirant efficacy of OP. Thus, virtual screening and structure-guided optimization yielded newly discovered agonists for manipulating crop abiotic stress tolerance and water use.

PubMed: 31649167
DOI: 10.1126/science.aaw8848

実験手法

X-RAY DIFFRACTION (2.35 Å)