6NUT

Ebola virus nucleoprotein - RNA complex

Summary for 6NUT

• Entry DOI: 10.2210/pdb6nut/pdb
• EMDB information: 0522
• Descriptor: Nucleoprotein, RNA (5'-R(P*AP*AP*AP*AP*AP*A)-3') (2 entities in total)
• Functional Keywords: rna-binding, nucleoprotein, nucleocapsid, capsid, viral protein-rna complex, viral protein/rna
• Biological source: Zaire ebolavirus (strain Mayinga-76) (ZEBOV); More
• Total number of polymer chains: 2
• Total formula weight: 52197.38

Authors

Kirchdoerfer, R.N.,Ward, A.B. (deposition date: 2019-02-01, release date: 2019-05-01, Last modification date: 2024-03-20)

Primary citation

Kirchdoerfer, R.N.,Saphire, E.O.,Ward, A.B.
Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex.
Acta Crystallogr.,Sect.F, 75:340-347, 2019

PubMed Abstract: Ebola virus is an emerging virus that is capable of causing a deadly disease in humans. Replication, transcription and packaging of the viral genome are carried out by the viral nucleocapsid. The nucleocapsid is a complex of the viral nucleoprotein, RNA and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. The 3.1 Å resolution single-particle cryo-electron microscopy structure of the nucleoprotein-RNA helical filament presented here resembles previous structures determined at lower resolution, while providing improved molecular details of protein-protein and protein-RNA interactions. The higher resolution of the structure presented here will facilitate the design and characterization of novel and specific Ebola virus therapeutics targeting the nucleocapsid.

PubMed: 31045563
DOI: 10.1107/S2053230X19004424

Experimental method

ELECTRON MICROSCOPY (3.1 Å)