6NUE

Small conformation of apo CRISPR_Csm complex

6NUE の概要

• エントリーDOI: 10.2210/pdb6nue/pdb
• EMDBエントリー: 0516 0517 0518 0519
• 分子名称: CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A), CRISPR system Cms protein Csm2, CRISPR type III-associated RAMP protein Csm3, ... (6 entities in total)
• 機能のキーワード: crispr, type iii-a, ssrnaase, ssdnase, hydrolase, transferase-rna complex, transferase/rna
• 由来する生物種: Streptococcus thermophilus; 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 309880.12

構造登録者

Zhang, K.,Pintilie, G.,Li, S.,Zhu, Y.,Chiu, W.,Huang, Z. (登録日: 2019-01-31, 公開日: 2019-03-13, 最終更新日: 2024-03-20)

主引用文献

Guo, M.,Zhang, K.,Zhu, Y.,Pintilie, G.D.,Guan, X.,Li, S.,Schmid, M.F.,Ma, Z.,Chiu, W.,Huang, Z.
Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.
Cell Res., 29:305-312, 2019

PubMed Abstract: The type III CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated genes) systems are bacterially encoded adaptive immune systems for defense against invading nucleic acids. They accomplish this task through the coordinated cleavage of invading substrates of single-stranded RNA and DNA (ssDNA and ssRNA) by the Csm (type III-A) or Cmr (type III-B) effector complexes. The ssRNA is complementarily bound to the CRISPR RNA (crRNA). However, the structural basis for the DNase and RNase activation of the Csm nucleoprotein complex is largely unknown. Here we report cryo-EM structures of the Csm-crRNA complex, with or without target ssRNA, at near-atomic resolution. Our cryo-EM maps allow us to build atomic models of the key macromolecular components, including Cas10, Csm2, Csm3, Csm4, crRNA and the invading ssRNA. Our structure resolves unambiguously the stoichiometry and tertiary structures of the Csm protein complex and the interactions between protein components and the crRNA/ssRNA. Interestingly, the new atomic structures of the Csm proteins presented here are similar to those of previously known Csm proteins in other species despite their low sequence similarity. Our combined structural and biochemical data suggest that ssRNA cleavage is preferentially carried out near its 5'-end, that the extent of interactions among the ssRNA, crRNA and the protein components regulates the DNase activity of the Csm complex, and that the 3' flanking sequence of ssRNA activates the Cas10 DNase activity allosterically.

PubMed: 30814678
DOI: 10.1038/s41422-019-0151-x

実験手法

ELECTRON MICROSCOPY (3.3 Å)