6NU4

Solution structure of the Arabidopsis thaliana RALF8 peptide

6NU4 の概要

• エントリーDOI: 10.2210/pdb6nu4/pdb
• NMR情報: BMRB: 30565
• 分子名称: Protein RALF-like 8 (1 entity in total)
• 機能のキーワード: rapid alkalinization factor-8, cysteine-rich plant peptide, plant growth, pollen tube generation, plant protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6248.08

構造登録者

Lee, W.,Markley, J.L.,Frederick, R.O.,Miyoshi, H.,Tonelli, M.,Cornilescu, G.,Cornilescu, C.,Sussman, M.R. (登録日: 2019-01-30, 公開日: 2019-05-08, 最終更新日: 2024-11-06)

主引用文献

Frederick, R.O.,Haruta, M.,Tonelli, M.,Lee, W.,Cornilescu, G.,Cornilescu, C.C.,Sussman, M.R.,Markley, J.L.
Function and solution structure of the Arabidopsis thaliana RALF8 peptide.
Protein Sci., 28:1115-1126, 2019

PubMed Abstract: We report the recombinant preparation from Escherichia coli cells of samples of two closely related, small, secreted cysteine-rich plant peptides: rapid alkalinization factor 1 (RALF1) and rapid alkalinization factor 8 (RALF8). Purified samples of the native sequence of RALF8 exhibited well-resolved nuclear magnetic resonance (NMR) spectra and also biological activity through interaction with a plant receptor kinase, cytoplasmic calcium mobilization, and in vivo root growth suppression. By contrast, RALF1 could only be isolated from inclusion bodies as a construct containing an N-terminal His-tag; its poorly resolved NMR spectrum was indicative of aggregation. We prepared samples of the RALF8 peptide labeled with N and C for NMR analysis and obtained near complete H, C, and N NMR assignments; determined the disulfide pairing of its four cysteine residues; and examined its solution structure. RALF8 is mostly disordered except for the two loops spanned by each of its two disulfide bridges.

PubMed: 31004454
DOI: 10.1002/pro.3628

実験手法

SOLUTION NMR