6NTR

Crystal Structure of Beta-barrel-like Protein of Domain of Unknown Function DUF1849 from Brucella abortus

「5UC2」から置き換えられました

6NTR の概要

• エントリーDOI: 10.2210/pdb6ntr/pdb
• 分子名称: ATP/GTP-binding site-containing protein A, GLYCEROL, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: beta barrel-like fold, beta-structure, cell envelope integrity, chicago center for functional annotation, midwest center for structural genomics, mcsg, psi-biology, unknown function
• 由来する生物種: Brucella abortus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 116932.68

構造登録者

Kim, Y.,Bigelow, L.,Endres, M.,Babnigg, G.,Crosson, S.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG) (登録日: 2019-01-30, 公開日: 2019-02-13, 最終更新日: 2024-10-16)

主引用文献

Herrou, J.,Willett, J.W.,Fiebig, A.,Czyz, D.M.,Cheng, J.X.,Ultee, E.,Briegel, A.,Bigelow, L.,Babnigg, G.,Kim, Y.,Crosson, S.
BrucellaPeriplasmic Protein EipB Is a Molecular Determinant of Cell Envelope Integrity and Virulence.
J.Bacteriol., 201:-, 2019

PubMed Abstract: The Gram-negative cell envelope is a remarkable structure with core components that include an inner membrane, an outer membrane, and a peptidoglycan layer in the periplasmic space between. Multiple molecular systems function to maintain integrity of this essential barrier between the interior of the cell and its surrounding environment. We show that a conserved DUF1849 family protein, EipB, is secreted to the periplasmic space of species, a monophyletic group of intracellular pathogens. In the periplasm, EipB folds into an unusual 14-stranded β-spiral structure that resembles the LolA and LolB lipoprotein delivery system, though the overall fold of EipB is distinct from LolA/LolB. Deletion of results in defects in cell envelope integrity and in maintenance of spleen colonization in a mouse model of infection. Transposon disruption of , which encodes a periplasmic protein containing tetratricopeptide repeats, is synthetically lethal with deletion. is a reported virulence determinant in , and our studies of deletion and overexpression strains provide evidence that this gene also contributes to cell envelope function. We conclude that and function in the periplasmic space to maintain cell envelope integrity, which facilitates survival in a mammalian host. species cause brucellosis, a global zoonosis. A gene encoding a conserved DUF1849-family protein, which we have named EipB, is present in all sequenced and several other genera in the class The manuscript provides the first functional and structural characterization of a DUF1849 protein. We show that EipB is secreted to the periplasm where it forms a spiral-shaped antiparallel β protein that is a determinant of cell envelope integrity and virulence in an animal model of disease. genetically interacts with , which also encodes a periplasmic protein. We propose that EipB and TtpA function as part of a system required for cell envelope homeostasis in select .

PubMed: 30936371
DOI: 10.1128/JB.00134-19

実験手法

X-RAY DIFFRACTION (2.101 Å)