6NTA

Modified ASL proline bound to Thermus thermophilus 70S (cognate)

これはPDB形式変換不可エントリーです。

6NTA の概要

• エントリーDOI: 10.2210/pdb6nta/pdb
• 分子名称: 16S rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (57 entities in total)
• 機能のキーワード: ribosome, proline, rna, asl
• 由来する生物種: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579); 詳細
• タンパク質・核酸の鎖数: 108
• 化学式量合計: 4392615.73

構造登録者

Hoffer, E.D.,Maehigashi, T.,Subaramanian, S.,Hong, S.,Dunham, C.M. (登録日: 2019-01-28, 公開日: 2020-10-14, 最終更新日: 2023-10-11)

主引用文献

Hoffer, E.,Hong, S.,Sunita, S.,Maehigashi, T.,Gonzalez Jnr, R.L.,Whitford, P.,Dunham, C.M.
Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon-anticodon pairing.
Elife, 9:-, 2020

PubMed Abstract: Modifications in the tRNA anticodon loop, adjacent to the three-nucleotide anticodon, influence translation fidelity by stabilizing the tRNA to allow for accurate reading of the mRNA genetic code. One example is the N1-methylguanosine modification at guanine nucleotide 37 (mG37) located in the anticodon loop andimmediately adjacent to the anticodon nucleotides 34, 35, 36. The absence of mG37 in tRNA causes +1 frameshifting on polynucleotide, slippery codons. Here, we report structures of the bacterial ribosome containing tRNA bound to either cognate or slippery codons to determine how the mG37 modification prevents mRNA frameshifting. The structures reveal that certain codon-anticodon contexts and the lack of mG37 destabilize interactions of tRNA with the P site of the ribosome, causing large conformational changes typically only seen during EF-G-mediated translocation of the mRNA-tRNA pairs. These studies provide molecular insights into how mG37 stabilizes the interactions of tRNA with the ribosome in the context of a slippery mRNA codon.

PubMed: 33016876
DOI: 10.7554/eLife.51898

実験手法

X-RAY DIFFRACTION (3.1 Å)