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6NRP

Putative short-chain dehydrogenase/reductase (SDR) from Acinetobacter baumannii

6NRP の概要
エントリーDOI10.2210/pdb6nrp/pdb
分子名称3-oxoacyl-ACP reductase FabG (2 entities in total)
機能のキーワードshort-chain dehydrogenase/reductase, sdr, rossmann fold, apo, oxidoreductase
由来する生物種Acinetobacter baumannii
タンパク質・核酸の鎖数4
化学式量合計114875.26
構造登録者
Cross, E.M.,Smith, K.M.,Shaw, K.I.,Aragao, D.,Forwood, J.K. (登録日: 2019-01-23, 公開日: 2019-02-27, 最終更新日: 2023-10-25)
主引用文献Cross, E.M.,Adams, F.G.,Waters, J.K.,Aragao, D.,Eijkelkamp, B.A.,Forwood, J.K.
Insights into Acinetobacter baumannii fatty acid synthesis 3-oxoacyl-ACP reductases.
Sci Rep, 11:7050-7050, 2021
Cited by
PubMed Abstract: Treatments for 'superbug' infections are the focus for innovative research, as drug resistance threatens human health and medical practices globally. In particular, Acinetobacter baumannii (Ab) infections are repeatedly reported as difficult to treat due to increasing antibiotic resistance. Therefore, there is increasing need to identify novel targets in the development of different antimicrobials. Of particular interest is fatty acid synthesis, vital for the formation of phospholipids, lipopolysaccharides/lipooligosaccharides, and lipoproteins of Gram-negative envelopes. The bacterial type II fatty acid synthesis (FASII) pathway is an attractive target for the development of inhibitors and is particularly favourable due to the differences from mammalian type I fatty acid synthesis. Discrete enzymes in this pathway include two reductase enzymes: 3-oxoacyl-acyl carrier protein (ACP) reductase (FabG) and enoyl-ACP reductase (FabI). Here, we investigate annotated FabG homologs, finding a low-molecular weight 3-oxoacyl-ACP reductase, as the most likely FASII FabG candidate, and high-molecular weight 3-oxoacyl-ACP reductase (HMwFabG), showing differences in structure and coenzyme preference. To date, this is the second bacterial high-molecular weight FabG structurally characterized, following FabG4 from Mycobacterium. We show that ΔAbHMwfabG is impaired for growth in nutrient rich media and pellicle formation. We also modelled a third 3-oxoacyl-ACP reductase, which we annotated as AbSDR. Despite containing residues for catalysis and the ACP coordinating motif, biochemical analyses showed limited activity against an acetoacetyl-CoA substrate in vitro. Inhibitors designed to target FabG proteins and thus prevent fatty acid synthesis may provide a platform for use against multidrug-resistant pathogens including A. baumannii.
PubMed: 33782435
DOI: 10.1038/s41598-021-86400-1
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.9 Å)
構造検証レポート
Validation report summary of 6nrp
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-13に公開中

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