6NRG

Crystal Structure of human PARP-1 ART domain bound to inhibitor UTT57

6NRG の概要

• エントリーDOI: 10.2210/pdb6nrg/pdb
• 関連するPDBエントリー: 6NRF
• 分子名称: Poly [ADP-ribose] polymerase 1, 2-{[3-fluoro-4-(1H-tetrazol-5-yl)phenyl]methyl}-3-hydroxy-1-benzofuran-7-carboxamide, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: parp-1, poly(adp-ribose) polymerase, parp inhibitor, parp1, artd1, transferase, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31071.30

構造登録者

Langelier, M.F.,Pascal, J.M. (登録日: 2019-01-23, 公開日: 2019-08-14, 最終更新日: 2023-10-11)

主引用文献

Velagapudi, U.K.,Langelier, M.F.,Delgado-Martin, C.,Diolaiti, M.E.,Bakker, S.,Ashworth, A.,Patel, B.A.,Shao, X.,Pascal, J.M.,Talele, T.T.
Design and Synthesis of Poly(ADP-ribose) Polymerase Inhibitors: Impact of Adenosine Pocket-Binding Motif Appendage to the 3-Oxo-2,3-dihydrobenzofuran-7-carboxamide on Potency and Selectivity.
J.Med.Chem., 62:5330-5357, 2019

PubMed Abstract: Poly(adenosine 5'-diphosphate-ribose) polymerase (PARP) inhibitors are a class of anticancer drugs that block the catalytic activity of PARP proteins. Optimization of our lead compound 1 (( Z)-2-benzylidene-3-oxo-2,3-dihydrobenzofuran-7-carboxamide; PARP-1 IC = 434 nM) led to a tetrazolyl analogue (51, IC = 35 nM) with improved inhibition. Isosteric replacement of the tetrazole ring with a carboxyl group (60, IC = 68 nM) gave a promising new lead, which was subsequently optimized to obtain analogues with potent PARP-1 IC values (4-197 nM). PARP enzyme profiling revealed that the majority of compounds are selective toward PARP-2 with IC values comparable to clinical inhibitors. X-ray crystal structures of the key inhibitors bound to PARP-1 illustrated the mode of interaction with analogue appendages extending toward the PARP-1 adenosine-binding pocket. Compound 81, an isoform-selective PARP-1/-2 (IC = 30 nM/2 nM) inhibitor, demonstrated selective cytotoxic effect toward breast cancer gene 1 ( BRCA1)-deficient cells compared to isogenic BRCA1-proficient cells.

PubMed: 31042381
DOI: 10.1021/acs.jmedchem.8b01709

実験手法

X-RAY DIFFRACTION (1.7 Å)