6NQI

Prp8 RH domain from C. merolae

6NQI の概要

• エントリーDOI: 10.2210/pdb6nqi/pdb
• 分子名称: Pre-mRNA splicing factor PRP8, BETA-MERCAPTOETHANOL (2 entities in total)
• 機能のキーワード: spliceosome, rnase h, splicing
• 由来する生物種: Cyanidioschyzon merolae (strain 10D) (Red alga)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49787.42

構造登録者

Garside, E.L.,MacMillan, A.M. (登録日: 2019-01-21, 公開日: 2019-05-22, 最終更新日: 2023-10-11)

主引用文献

Garside, E.L.,Whelan, T.A.,Stark, M.R.,Rader, S.D.,Fast, N.M.,MacMillan, A.M.
Prp8 in a Reduced Spliceosome Lacks a Conserved Toggle that Correlates with Splicing Complexity across Diverse Taxa.
J.Mol.Biol., 431:2543-2553, 2019

PubMed Abstract: Conformational rearrangements are critical to regulating the assembly and activity of the spliceosome. The spliceosomal protein Prp8 undergoes multiple conformational changes during the course of spliceosome assembly, activation, and catalytic activity. Most of these rearrangements of Prp8 involve the disposition of the C-terminal Jab-MPN and RH domains with respect to the core of Prp8. Here we use x-ray structural analysis to show that a previously characterized and highly conserved β-hairpin structure in the RH domain that acts as a toggle in the spliceosome is absent in Prp8 from the reduced spliceosome of the red alga Cyanidioschyzon merolae. Using comparative sequence analysis, we show that the presence or absence of this hairpin corresponds to the presence or absence of protein partners that interact with this hairpin as observed by x-ray and cryo-EM studies. The presence of the toggle correlates with increasing intron number suggesting a role in the regulation of splicing.

PubMed: 31078556
DOI: 10.1016/j.jmb.2019.04.047

実験手法

X-RAY DIFFRACTION (2.75 Å)