6NQD

Cryo-EM structure of T/F100 SOSIP.664 HIV-1 Env trimer in complex with 8ANC195 Fab

6NQD の概要

• エントリーDOI: 10.2210/pdb6nqd/pdb
• EMDBエントリー: 0485
• 分子名称: T/F100 Env gp120, 2-acetamido-2-deoxy-beta-D-glucopyranose, T/F100 Env gp41, ... (10 entities in total)
• 機能のキーワード: hiv-1, env, trimer, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 401996.83

構造登録者

Fang, Q.,Rossmann, M.G. (登録日: 2019-01-21, 公開日: 2019-03-06, 最終更新日: 2024-10-16)

主引用文献

Ananthaswamy, N.,Fang, Q.,AlSalmi, W.,Jain, S.,Chen, Z.,Klose, T.,Sun, Y.,Liu, Y.,Mahalingam, M.,Chand, S.,Tovanabutra, S.,Robb, M.L.,Rossmann, M.G.,Rao, V.B.
A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer.
Nat Commun, 10:873-873, 2019

PubMed Abstract: The envelope protein of human immunodeficiency virus-1 (HIV-1) and its fusion peptide are essential for cell entry and vaccine design. Here, we describe the 3.9-Å resolution structure of an envelope protein trimer from a very early transmitted founder virus (CRF01_AE T/F100) complexed with Fab from the broadly neutralizing antibody (bNAb) 8ANC195. The overall T/F100 trimer structure is similar to other reported "closed" state prefusion trimer structures. In contrast, the fusion peptide, which is exposed to solvent in reported closed structures, is sequestered (buried) in the hydrophobic core of the T/F100 trimer. A buried conformation has previously been observed in "open" state structures formed after CD4 receptor binding. The T/F100 trimer binds poorly to bNAbs including the fusion peptide-specific bNAbs PGT151 and VRC34.01. The T/F100 structure might represent a prefusion state, intermediate between the closed and open states. These observations are relevant to mechanisms of HIV-1 transmission and vaccine design.

PubMed: 30787293
DOI: 10.1038/s41467-019-08825-7

実験手法

ELECTRON MICROSCOPY (3.9 Å)