6NON

Structure of Cyanthece apo McdA

6NON の概要

• エントリーDOI: 10.2210/pdb6non/pdb
• 分子名称: Cobyrinic acid ac-diamide synthase, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: nonspecific dna binding protein, walker box, para-like, carboxysome, mcda, mcdb, dna binding protein
• 由来する生物種: Cyanothece
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59102.88

構造登録者

Schumacher, M.A. (登録日: 2019-01-16, 公開日: 2019-04-24, 最終更新日: 2024-11-13)

主引用文献

Schumacher, M.A.,Henderson, M.,Zhang, H.
Structures of maintenance of carboxysome distribution Walker-box McdA and McdB adaptor homologs.
Nucleic Acids Res., 47:5950-5962, 2019

PubMed Abstract: Carboxysomes, protein-coated organelles in cyanobacteria, are important in global carbon fixation. However, these organelles are present at low copy in each cell and hence must be segregated to ensure transmission from one generation to the next. Recent studies revealed that a DNA partition-like ParA-ParB system mediates carboxysome maintenance, called McdA-McdB. Here, we describe the first McdA and McdB homolog structures. McdA is similar to partition ParA Walker-box proteins, but lacks the P-loop signature lysine involved in ATP binding. Strikingly, a McdA-ATP structure shows that a lysine distant from the P-loop and conserved in McdA homologs, enables ATP-dependent nucleotide sandwich dimer formation. Similar to partition ParA proteins this ATP-bound form binds nonspecific-DNA. McdB, which we show directly binds McdA, harbors a unique fold and appears to form higher-order oligomers like partition ParB proteins. Thus, our data reveal a new signature motif that enables McdA dimer formation and indicates that, similar to DNA segregation, carboxysome maintenance systems employ Walker-box proteins as DNA-binding motors while McdB proteins form higher order oligomers, which could function as adaptors to link carboxysomes and provide for stable transport by the McdA proteins.

PubMed: 31106331
DOI: 10.1093/nar/gkz314

実験手法

X-RAY DIFFRACTION (2.68 Å)