6NOM

NMR solution structure of Pisum sativum defensin 2 (Psd2) provides evidence for the presence of hydrophobic surface clusters

6NOM の概要

• エントリーDOI: 10.2210/pdb6nom/pdb
• NMR情報: BMRB: 30561
• 分子名称: Defensin-2 (1 entity in total)
• 機能のキーワード: psd1, psd2, plant defensins, antimicrobial peptides, antimicrobial protein
• 由来する生物種: Pisum sativum (Garden pea)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5414.16

構造登録者

Pinheiro-Aguiar, R.,Amaral, V.S.G.,Bastos, I.,Kurtenbach, E.,Almeida, F.C.L. (登録日: 2019-01-16, 公開日: 2019-08-21, 最終更新日: 2024-10-23)

主引用文献

Pinheiro-Aguiar, R.,do Amaral, V.S.G.,Pereira, I.B.,Kurtenbach, E.,Almeida, F.C.L.
Nuclear magnetic resonance solution structure of Pisum sativum defensin 2 provides evidence for the presence of hydrophobic surface-clusters.
Proteins, 88:242-246, 2020

PubMed Abstract: Pisum sativum defensin 2 (Psd2) is a small (4.7 kDa) antifungal peptide whose structure is held together by four conserved disulfide bridges. Psd2 shares the cysteine-stabilized alpha-beta (CSαβ) fold, which lacks a regular hydrophobic core. All hydrophobic residues are exposed to the surface, except for leucine 6. They are clustered in the surface formed by two loops, between β1 and α-helix and β2 and β3 sheets. The observation of surface hydrophobic clusters reveals a remarkable evolution of the CSαβ fold to expose and reorganize hydrophobic residues, which facilitates creating versatile binding sites.

PubMed: 31294889
DOI: 10.1002/prot.25783

実験手法

SOLUTION NMR