6NJQ

Structure of TBP-Hoogsteen containing DNA complex

6NJQ の概要

• エントリーDOI: 10.2210/pdb6njq/pdb
• 分子名称: TATA-box-binding protein 1, DNA (5'-D(*GP*CP*TP*AP*TP*AP*AP*AP*CP*GP*GP*GP*CP*A)-3'), DNA (5'-D(*TP*GP*CP*CP*CP*GP*TP*TP*TP*AP*TP*AP*GP*C)-3'), ... (4 entities in total)
• 機能のキーワード: tbp, hoogsteen, protein-dna, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 66264.64

構造登録者

Schumacher, M.A.,Stelling, A. (登録日: 2019-01-04, 公開日: 2019-10-30, 最終更新日: 2023-10-11)

主引用文献

Stelling, A.L.,Liu, A.Y.,Zeng, W.,Salinas, R.,Schumacher, M.A.,Al-Hashimi, H.M.
Infrared Spectroscopic Observation of a G-C+Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions.
Angew.Chem.Int.Ed.Engl., 58:12010-12013, 2019

PubMed Abstract: Hoogsteen DNA base pairs (bps) are an alternative base pairing to canonical Watson-Crick bps and are thought to play important biochemical roles. Hoogsteen bps have been reported in a handful of X-ray structures of protein-DNA complexes. However, there are several examples of Hoogsteen bps in crystal structures that form Watson-Crick bps when examined under solution conditions. Furthermore, Hoogsteen bps can sometimes be difficult to resolve in DNA:protein complexes by X-ray crystallography due to ambiguous electron density and by solution-state NMR spectroscopy due to size limitations. Here, using infrared spectroscopy, we report the first direct solution-state observation of a Hoogsteen (G-C ) bp in a DNA:protein complex under solution conditions with specific application to DNA-bound TATA-box binding protein. These results support a previous assignment of a G-C Hoogsteen bp in the complex, and indicate that Hoogsteen bps do indeed exist under solution conditions in DNA:protein complexes.

PubMed: 31268220
DOI: 10.1002/anie.201902693

実験手法

X-RAY DIFFRACTION (2.75 Å)