6NJ2

thermostable carbonic anhydrase II variant with tetrazine 2.0 at site 186

6NJ2 の概要

• エントリーDOI: 10.2210/pdb6nj2/pdb
• 分子名称: Carbonic anhydrase 2, ZINC ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: genetic code expansion, thermostability, protein engineering, lyase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61083.04

構造登録者

Kean, K.M.,Karplus, P.A. (登録日: 2019-01-02, 公開日: 2019-10-02, 最終更新日: 2023-10-11)

主引用文献

Bednar, R.M.,Golbek, T.W.,Kean, K.M.,Brown, W.J.,Jana, S.,Baio, J.E.,Karplus, P.A.,Mehl, R.A.
Immobilization of Proteins with Controlled Load and Orientation.
ACS Appl Mater Interfaces, 11:36391-36398, 2019

PubMed Abstract: Biomaterials based on immobilized proteins are key elements of many biomedical and industrial technologies. However, applications are limited by an inability to precisely construct materials of high homogeneity and defined content. We present here a general "protein-limited immobilization" strategy by combining the rapid, bioorthogonal, and biocompatible properties of a tetrazine-strained -cyclooctene reaction with genetic code expansion to site-specifically place the tetrazine into a protein. For the first time, we use this strategy to immobilize defined amounts of oriented proteins onto beads and flat surfaces in under 5 min at submicromolar concentrations without compromising activity. This approach opens the door to generating and studying diverse protein-based biomaterials that are much more precisely defined and characterized, providing a greater ability to engineer properties across a wide range of applications.

PubMed: 31525993
DOI: 10.1021/acsami.9b12746

実験手法

X-RAY DIFFRACTION (1.5 Å)