6NHT

Single particle reconstruction of the symmetric core an engineered protein scaffold

6NHT の概要

• エントリーDOI: 10.2210/pdb6nht/pdb
• EMDBエントリー: 9373 9374
• 分子名称: DARP14 - Subunit A with DARPin, DARP14 - Subunit B (2 entities in total)
• 機能のキーワード: protein engineering, symmetric scaffold, small protein cryo-em, display platform, biosynthetic protein
• 由来する生物種: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3); 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 588769.13

構造登録者

Liu, Y.,Huynh, D.,Yeates, T.O. (登録日: 2018-12-23, 公開日: 2019-05-08, 最終更新日: 2024-03-20)

主引用文献

Liu, Y.,Huynh, D.T.,Yeates, T.O.
A 3.8 angstrom resolution cryo-EM structure of a small protein bound to an imaging scaffold.
Nat Commun, 10:1864-1864, 2019

PubMed Abstract: Proteins smaller than about 50 kDa are currently too small to be imaged at high resolution by cryo-electron microscopy (cryo-EM), leaving most protein molecules in the cell beyond the reach of this powerful structural technique. Here we use a designed protein scaffold to bind and symmetrically display 12 copies of a small 26 kDa protein, green fluorescent protein (GFP). We show that the bound cargo protein is held rigidly enough to visualize it at a resolution of 3.8 Å by cryo-EM, where specific structural features of the protein are visible. The designed scaffold is modular and can be modified through modest changes in its amino acid sequence to bind and display diverse proteins for imaging, thus providing a general method to break through the lower size limitation in cryo-EM.

PubMed: 31015551
DOI: 10.1038/s41467-019-09836-0

実験手法

ELECTRON MICROSCOPY (2.9 Å)