6NC6

Lipid II flippase MurJ, inward closed conformation

6NC6 の概要

• エントリーDOI: 10.2210/pdb6nc6/pdb
• 分子名称: Lipid II flippase MurJ, SODIUM ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: transporter, flippase, peptidoglycan, cell wall, transport protein
• 由来する生物種: Thermosipho africanus (strain TCF52B)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 108998.98

構造登録者

Kuk, A.C.Y.,Lee, S.-Y. (登録日: 2018-12-10, 公開日: 2019-04-17, 最終更新日: 2023-10-11)

主引用文献

Kuk, A.C.Y.,Hao, A.,Guan, Z.,Lee, S.Y.
Visualizing conformation transitions of the Lipid II flippase MurJ.
Nat Commun, 10:1736-1736, 2019

PubMed Abstract: The biosynthesis of many polysaccharides, including bacterial peptidoglycan and eukaryotic N-linked glycans, requires transport of lipid-linked oligosaccharide (LLO) precursors across the membrane by specialized flippases. MurJ is the flippase for the lipid-linked peptidoglycan precursor Lipid II, a key player in bacterial cell wall synthesis, and a target of recently discovered antibacterials. However, the flipping mechanism of LLOs including Lipid II remains poorly understood due to a dearth of structural information. Here we report crystal structures of MurJ captured in inward-closed, inward-open, inward-occluded and outward-facing conformations. Together with mutagenesis studies, we elucidate the conformational transitions in MurJ that mediate lipid flipping, identify the key ion for function, and provide a framework for the development of inhibitors.

PubMed: 30988294
DOI: 10.1038/s41467-019-09658-0

実験手法

X-RAY DIFFRACTION (3.2 Å)