6N8H

RNA Duplex containing the internal loop 5'-GCAU/3'-UACG

6N8H の概要

• エントリーDOI: 10.2210/pdb6n8h/pdb
• NMR情報: BMRB: 30547
• 分子名称: RNA (5'-R(*CP*GP*GP*GP*CP*AP*UP*CP*CP*G)-3') (1 entity in total)
• 機能のキーワード: duplex, internal loop, gu wobble, rna, ca pair
• 由来する生物種: unidentified
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6383.93

構造登録者

Berger, K.D.,Kennedy, S.D.,Turner, D.H. (登録日: 2018-11-29, 公開日: 2019-02-13, 最終更新日: 2024-05-01)

主引用文献

Berger, K.D.,Kennedy, S.D.,Turner, D.H.
Nuclear Magnetic Resonance Reveals That GU Base Pairs Flanking Internal Loops Can Adopt Diverse Structures.
Biochemistry, 58:1094-1108, 2019

PubMed Abstract: RNA thermodynamics play an important role in determining the two- and three-dimensional structures of RNA. Internal loops of the sequence 5'-GMNU/3'-UNMG are relatively unstable thermodynamically. Here, five duplexes with GU-flanked 2 × 2 nucleotide internal loops were structurally investigated to reveal determinants of their instability. The following internal loops were investigated: 5'-GCAU/3'-UACG, 5'-UUCG/3'-GCUU, 5'-GCUU/3'-UUCG, 5'-GUCU/3'-UCUG, and 5'-GCCU/3'-UCCG. Two-dimensional nuclear magnetic resonance spectra indicate the absence of GU wobble base pairing in 5'-GCUU/3'-UUCG, 5'-GUCU/3'-UCUG, and 5'-GCCU/3'-UCCG. The 5'-GCUU/3'-UUCG loop has an unusual conformation of the GU base pairs, in which U's O2 carbonyl forms a bifurcated hydrogen bond with G's amino and imino protons. The internal loop of 5'-GUCU/3'-UCUG displays a shifted configuration in which GC pairs flank a U-U pair and several U's are in fast exchange between positions inside and outside the helix. In contrast, 5'-GCAU/3'-UACG and 5'-UUCG/3'-GCUU both have the expected GU wobble base pairs flanking the internal loop. Evidently, GU base pairs flanking internal loops are more likely to display atypical structures relative to Watson-Crick base pairs flanking internal loops. This appears to be more likely when the G of the GU pair is 5' to the loop. Such unusual structures could serve as recognition elements for biological function and as benchmarks for structure prediction methods.

PubMed: 30702283
DOI: 10.1021/acs.biochem.8b01027

実験手法

SOLUTION NMR