6N86

Resistance to inhibitors of cholinesterase 8A (Ric8A) protein

6N86 の概要

• エントリーDOI: 10.2210/pdb6n86/pdb
• 分子名称: Synembryn-A (1 entity in total)
• 機能のキーワード: ric8a, armadillo repeat, g alpha, chaperone
• 由来する生物種: Bos taurus (Bovine)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57639.15

構造登録者

Srivastava, D.,Gakhar, L.,Artemyev, N.O. (登録日: 2018-11-28, 公開日: 2019-07-10, 最終更新日: 2024-03-13)

主引用文献

Srivastava, D.,Gakhar, L.,Artemyev, N.O.
Structural underpinnings of Ric8A function as a G-protein alpha-subunit chaperone and guanine-nucleotide exchange factor.
Nat Commun, 10:3084-3084, 2019

PubMed Abstract: Resistance to inhibitors of cholinesterase 8A (Ric8A) is an essential regulator of G protein α-subunits (Gα), acting as a guanine nucleotide exchange factor and a chaperone. We report two crystal structures of Ric8A, one in the apo form and the other in complex with a tagged C-terminal fragment of Gα. These structures reveal two principal domains of Ric8A: an armadillo-fold core and a flexible C-terminal tail. Additionally, they show that the Gα C-terminus binds to a highly-conserved patch on the concave surface of the Ric8A armadillo-domain, with selectivity determinants residing in the Gα sequence. Biochemical analysis shows that the Ric8A C-terminal tail is critical for its stability and function. A model of the Ric8A/Gα complex derived from crosslinking mass spectrometry and molecular dynamics simulations suggests that the Ric8A C-terminal tail helps organize the GTP-binding site of Gα. This study lays the groundwork for understanding Ric8A function at the molecular level.

PubMed: 31300652
DOI: 10.1038/s41467-019-11088-x

実験手法

X-RAY DIFFRACTION (3.9 Å)