6N7X

S. cerevisiae U1 snRNP

「5UZ5」から置き換えられました

6N7X の概要

• エントリーDOI: 10.2210/pdb6n7x/pdb
• EMDBエントリー: 8622
• 分子名称: U1 small nuclear ribonucleoprotein 70 kDa homolog, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, ... (16 entities in total)
• 機能のキーワード: pre-mrna splicing, u1 snrnp, alternative splicing, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 694678.06

構造登録者

Li, X.,Liu, S.,Jiang, J.,Zhang, L.,Espinosa, S.,Hill, R.C.,Hansen, K.C.,Zhou, Z.H.,Zhao, R. (登録日: 2018-11-28, 公開日: 2019-07-24, 最終更新日: 2024-03-13)

主引用文献

Li, X.,Liu, S.,Jiang, J.,Zhang, L.,Espinosa, S.,Hill, R.C.,Hansen, K.C.,Zhou, Z.H.,Zhao, R.
CryoEM structure of Saccharomyces cerevisiae U1 snRNP offers insight into alternative splicing.
Nat Commun, 8:1035-1035, 2017

PubMed Abstract: U1 snRNP plays a critical role in 5'-splice site recognition and is a frequent target of alternative splicing factors. These factors transiently associate with human U1 snRNP and are not amenable for structural studies, while their Saccharomyces cerevisiae (yeast) homologs are stable components of U1 snRNP. Here, we report the cryoEM structure of yeast U1 snRNP at 3.6 Å resolution with atomic models for ten core proteins, nearly all essential domains of its RNA, and five stably associated auxiliary proteins. The foot-shaped yeast U1 snRNP contains a core in the "ball-and-toes" region architecturally similar to the human U1 snRNP. All auxiliary proteins are in the "arch-and-heel" region and connected to the core through the Prp42/Prp39 paralogs. Our demonstration that homodimeric human PrpF39 directly interacts with U1C-CTD, mirroring yeast Prp42/Prp39, supports yeast U1 snRNP as a model for understanding how transiently associated auxiliary proteins recruit human U1 snRNP in alternative splicing.

PubMed: 29051543
DOI: 10.1038/s41467-017-01241-9

実験手法

ELECTRON MICROSCOPY (3.6 Å)