6N4N

Crystal structure of the designed protein DNCR2/danoprevir/NS3a complex

6N4N の概要

• エントリーDOI: 10.2210/pdb6n4n/pdb
• 分子名称: NS3 protease, Rosetta-designed danoprevir/NS3a complex reader 2, ZINC ION, ... (6 entities in total)
• 機能のキーワード: rosetta design, procisir, ns3a, danoprevir, de novo protein-hydrolase-inhibitor complex, de novo protein/hydrolase/inhibitor
• 由来する生物種: Hepacivirus C (Hepatitis C virus); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 95356.75

構造登録者

Wang, Z.,Foight, G.W.,Baker, D.,Maly, D.J. (登録日: 2018-11-19, 公開日: 2019-09-11, 最終更新日: 2023-10-11)

主引用文献

Foight, G.W.,Wang, Z.,Wei, C.T.,Jr Greisen, P.,Warner, K.M.,Cunningham-Bryant, D.,Park, K.,Brunette, T.J.,Sheffler, W.,Baker, D.,Maly, D.J.
Multi-input chemical control of protein dimerization for programming graded cellular responses.
Nat.Biotechnol., 37:1209-1216, 2019

PubMed Abstract: Chemical and optogenetic methods for post-translationally controlling protein function have enabled modulation and engineering of cellular functions. However, most of these methods only confer single-input, single-output control. To increase the diversity of post-translational behaviors that can be programmed, we built a system based on a single protein receiver that can integrate multiple drug inputs, including approved therapeutics. Our system translates drug inputs into diverse outputs using a suite of engineered reader proteins to provide variable dimerization states of the receiver protein. We show that our single receiver protein architecture can be used to program a variety of cellular responses, including graded and proportional dual-output control of transcription and mammalian cell signaling. We apply our tools to titrate the competing activities of the Rac and Rho GTPases to control cell morphology. Our versatile tool set will enable researchers to post-translationally program mammalian cellular processes and to engineer cell therapies.

PubMed: 31501561
DOI: 10.1038/s41587-019-0242-8

実験手法

X-RAY DIFFRACTION (2.29 Å)