6N38

Structure of the type VI secretion system TssK-TssF-TssG baseplate subcomplex revealed by cryo-electron microscopy - full map sharpened

6N38 の概要

• エントリーDOI: 10.2210/pdb6n38/pdb
• EMDBエントリー: 9341 9342 9343
• 分子名称: Putative type VI secretion protein, Unassigned protein, ... (4 entities in total)
• 機能のキーワード: type vi secretion system, enteroaggregative e. coli, protein secretion, baseplate, structural genomics, seattle structural genomics center for infectious disease, ssgcid, protein transport
• 由来する生物種: Escherichia coli O44:H18 (strain 042 / EAEC); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 389978.64

構造登録者

Park, Y.J.,Lacourse, K.D.,Cambillau, C.,Seattle Structural Genomics Center for Infectious Disease (SSGCID),DiMaio, F.,Mougous, J.D.,Veesler, D. (登録日: 2018-11-14, 公開日: 2018-12-26, 最終更新日: 2024-03-20)

主引用文献

Park, Y.J.,Lacourse, K.D.,Cambillau, C.,DiMaio, F.,Mougous, J.D.,Veesler, D.
Structure of the type VI secretion system TssK-TssF-TssG baseplate subcomplex revealed by cryo-electron microscopy.
Nat Commun, 9:5385-5385, 2018

PubMed Abstract: Type VI secretion systems (T6SSs) translocate effectors into target cells and are made of a contractile sheath and a tube docked onto a multi-protein transmembrane complex via a baseplate. Although some information is available about the mechanisms of tail contraction leading to effector delivery, the detailed architecture and function of the baseplate remain unknown. Here, we report the 3.7 Å resolution cryo-electron microscopy reconstruction of an enteroaggregative Escherichia coli baseplate subcomplex assembled from TssK, TssF and TssG. The structure reveals two TssK trimers interact with a locally pseudo-3-fold symmetrical complex comprising two copies of TssF and one copy of TssG. TssF and TssG are structurally related to each other and to components of the phage T4 baseplate and of the type IV secretion system, strengthening the evolutionary relationships among these macromolecular machines. These results, together with bacterial two-hybrid assays, provide a structural framework to understand the T6SS baseplate architecture.

PubMed: 30568167
DOI: 10.1038/s41467-018-07796-5

実験手法

ELECTRON MICROSCOPY (3.7 Å)