6MZZ

Fluoroacetate dehalogenase, room temperature structure, using first 1 degree of total 3 degree oscillation

6MZZ の概要

• エントリーDOI: 10.2210/pdb6mzz/pdb
• 関連するPDBエントリー: 6MUY
• 分子名称: Fluoroacetate dehalogenase, CALCIUM ION (3 entities in total)
• 機能のキーワード: dehalogenase, defluorinase, hydrolase
• 由来する生物種: Rhodopseudomonas palustris CGA009
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68187.40

構造登録者

Finke, A.D.,Wierman, J.L.,Pare-Labrosse, O.,Sarrachini, A.,Besaw, J.,Mehrabi, P.,Gruner, S.M.,Miller, R.J.D. (登録日: 2018-11-06, 公開日: 2019-03-27, 最終更新日: 2023-10-11)

主引用文献

Wierman, J.L.,Pare-Labrosse, O.,Sarracini, A.,Besaw, J.E.,Cook, M.J.,Oghbaey, S.,Daoud, H.,Mehrabi, P.,Kriksunov, I.,Kuo, A.,Schuller, D.J.,Smith, S.,Ernst, O.P.,Szebenyi, D.M.E.,Gruner, S.M.,Miller, R.J.D.,Finke, A.D.
Fixed-target serial oscillation crystallography at room temperature.
IUCrJ, 6:305-316, 2019

PubMed Abstract: A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1-5° of data per crystal at room temperature with fast (10° s) oscillation rates and translation times, giving a crystal-data collection rate of 2.5 Hz. Partial datasets collected by the oscillation method at a storage-ring source provide more complete data per crystal than still images, dramatically lowering the total number of crystals needed for a complete dataset suitable for structure solution and refinement - up to two orders of magnitude fewer being required. Thus, this method is particularly well suited to instances where crystal quantities are low. It is demonstrated, through comparison of first and last oscillation images of two systems, that dose and the effects of radiation damage can be minimized through fast rotation and low angular sweeps for each crystal.

PubMed: 30867928
DOI: 10.1107/S2052252519001453

実験手法

X-RAY DIFFRACTION (1.8 Å)