6MZX

Cryo-EM structure of the HO BMC shell: Icosahedral reconstruction (main population)

これはPDB形式変換不可エントリーです。

6MZX の概要

• エントリーDOI: 10.2210/pdb6mzx/pdb
• 関連するPDBエントリー: 6MZu 6MZv 6MZY 6N06 6N07 6N09 6N0F 6N0g
• EMDBエントリー: 9296 9307 9308 9309 9310 9311 9312 9313 9314 9315
• 分子名称: Ethanolamine utilization protein EutN/carboxysome structural protein Ccml, Microcompartments protein HO-5815, Microcompartments protein HO-5816 (3 entities in total)
• 機能のキーワード: microcompartment, shell, compartmentalization, bmc fold, structural protein, virus like particle
• 由来する生物種: Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 116434.90

構造登録者

Greber, B.J.,Sutter, M.,Kerfeld, C.A. (登録日: 2018-11-06, 公開日: 2019-03-13, 最終更新日: 2024-03-13)

主引用文献

Greber, B.J.,Sutter, M.,Kerfeld, C.A.
The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.
Structure, 27:749-763.e4, 2019

PubMed Abstract: Bacterial microcompartments (BMCs) are composed of an enzymatic core encapsulated by a selectively permeable protein shell that enhances catalytic efficiency. Many pathogenic bacteria derive competitive advantages from their BMC-based catabolism, implicating BMCs as drug targets. BMC shells are of interest for bioengineering due to their diverse and selective permeability properties and because they self-assemble. A complete understanding of shell composition and organization is a prerequisite for biotechnological applications. Here, we report the cryoelectron microscopy structure of a BMC shell at 3.0-Å resolution, using an image-processing strategy that allowed us to determine the previously uncharacterized structural details of the interactions formed by the BMC-T and BMC-T shell subunits in the context of the assembled shell. We found unexpected structural plasticity among these interactions, resulting in distinct shell populations assembled from varying numbers of the BMC-T and BMC-T subunits. We discuss the implications of these findings on shell assembly and function.

PubMed: 30833088
DOI: 10.1016/j.str.2019.01.017

実験手法

ELECTRON MICROSCOPY (3 Å)