6MXQ

Solution structure of a c-JUN 5' UTR stem-loop associated with specialized cap-dependent translation initiation

Summary for 6MXQ

• Entry DOI: 10.2210/pdb6mxq/pdb
• NMR Information: BMRB: 30533
• Descriptor: RNA C-JUN TL (1 entity in total)
• Functional Keywords: rna, c-jun, mrna, eif3, cap-dependent, specialized translation initiation
• Biological source: Homo sapiens
• Total number of polymer chains: 1
• Total formula weight: 15523.18

Authors

Walker, M.,Shortridge, M.,Varani, G. (deposition date: 2018-10-31, release date: 2020-05-06, Last modification date: 2024-05-01)

Primary citation

Walker, M.J.,Shortridge, M.D.,Albin, D.D.,Cominsky, L.Y.,Varani, G.
Structure of the RNA Specialized Translation Initiation Element that Recruits eIF3 to the 5'-UTR of c-Jun.
J.Mol.Biol., 432:1841-1855, 2020

PubMed Abstract: Specialized translation initiation is a novel form of regulation of protein synthesis, whereby RNA structures within the 5'-UTR regulate translation rates of specific mRNAs. Similar to internal ribosome entry sites (IRESs), specialized translation initiation requires the recruitment of eukaryotic initiation factor 3 (eIF3), but also requires cap recognition by eIF3d, a new 5'-mGTP recognizing protein. How these RNA structures mediate eIF3 recruitment to affect translation of specific mRNAs remains unclear. Here, we report the nuclear magnetic resonance (NMR) structure of a stem-loop within the c-JUN 5' UTR recognized by eIF3 and essential for specialized translation initiation of this well-known oncogene. The structure exhibits similarity to eIF3 recognizing motifs found in hepatitis C virus (HCV)-like IRESs, suggesting mechanistic similarities. This work establishes the RNA structural features involved in c-JUN specialized translation initiation and provides a basis to search for small molecule inhibitors of aberrant expression of the proto-oncogenic c-JUN.

PubMed: 31953146
DOI: 10.1016/j.jmb.2020.01.001

Experimental method

SOLUTION NMR