6MV0
CO-bound Sperm Whale Myoglobin, room temperature structure solved by serial 5degree oscillation crystallography
Summary for 6MV0
| Entry DOI | 10.2210/pdb6mv0/pdb |
| Related | 6MUH 6MUY 6MUZ |
| Descriptor | Myoglobin, PROTOPORPHYRIN IX CONTAINING FE, CARBON MONOXIDE, ... (5 entities in total) |
| Functional Keywords | oxygen transporter, myoglobin, transport protein |
| Biological source | Physeter catodon (Sperm whale) |
| Total number of polymer chains | 1 |
| Total formula weight | 18106.71 |
| Authors | Finke, A.D.,Wierman, J.L.,Pare-Labrosse, O.,Sarrachini, A.,Besaw, J.,Mehrabi, P.,Gruner, S.M.,Miller, R.J.D. (deposition date: 2018-10-24, release date: 2019-03-27, Last modification date: 2023-10-11) |
| Primary citation | Wierman, J.L.,Pare-Labrosse, O.,Sarracini, A.,Besaw, J.E.,Cook, M.J.,Oghbaey, S.,Daoud, H.,Mehrabi, P.,Kriksunov, I.,Kuo, A.,Schuller, D.J.,Smith, S.,Ernst, O.P.,Szebenyi, D.M.E.,Gruner, S.M.,Miller, R.J.D.,Finke, A.D. Fixed-target serial oscillation crystallography at room temperature. IUCrJ, 6:305-316, 2019 Cited by PubMed Abstract: A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1-5° of data per crystal at room temperature with fast (10° s) oscillation rates and translation times, giving a crystal-data collection rate of 2.5 Hz. Partial datasets collected by the oscillation method at a storage-ring source provide more complete data per crystal than still images, dramatically lowering the total number of crystals needed for a complete dataset suitable for structure solution and refinement - up to two orders of magnitude fewer being required. Thus, this method is particularly well suited to instances where crystal quantities are low. It is demonstrated, through comparison of first and last oscillation images of two systems, that dose and the effects of radiation damage can be minimized through fast rotation and low angular sweeps for each crystal. PubMed: 30867928DOI: 10.1107/S2052252519001453 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.97 Å) |
Structure validation
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