6MUY

Fluoroacetate dehalogenase, room temperature structure solved by serial 3 degree oscillation crystallography

Summary for 6MUY

• Entry DOI: 10.2210/pdb6muy/pdb
• Related: 6MUH
• Descriptor: Fluoroacetate dehalogenase, CALCIUM ION (3 entities in total)
• Functional Keywords: dehalogenase, defluorinase, hydrolase
• Biological source: Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
• Total number of polymer chains: 2
• Total formula weight: 68187.40

Authors

Finke, A.D.,Wierman, J.L.,Pare-Labrosse, O.,Sarrachini, A.,Besaw, J.,Mehrabi, P.,Gruner, S.M.,Miller, R.J.D. (deposition date: 2018-10-24, release date: 2019-03-27, Last modification date: 2023-10-11)

Primary citation

Wierman, J.L.,Pare-Labrosse, O.,Sarracini, A.,Besaw, J.E.,Cook, M.J.,Oghbaey, S.,Daoud, H.,Mehrabi, P.,Kriksunov, I.,Kuo, A.,Schuller, D.J.,Smith, S.,Ernst, O.P.,Szebenyi, D.M.E.,Gruner, S.M.,Miller, R.J.D.,Finke, A.D.
Fixed-target serial oscillation crystallography at room temperature.
IUCrJ, 6:305-316, 2019

PubMed Abstract: A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1-5° of data per crystal at room temperature with fast (10° s) oscillation rates and translation times, giving a crystal-data collection rate of 2.5 Hz. Partial datasets collected by the oscillation method at a storage-ring source provide more complete data per crystal than still images, dramatically lowering the total number of crystals needed for a complete dataset suitable for structure solution and refinement - up to two orders of magnitude fewer being required. Thus, this method is particularly well suited to instances where crystal quantities are low. It is demonstrated, through comparison of first and last oscillation images of two systems, that dose and the effects of radiation damage can be minimized through fast rotation and low angular sweeps for each crystal.

PubMed: 30867928
DOI: 10.1107/S2052252519001453

Experimental method

X-RAY DIFFRACTION (1.8 Å)