6MTC

Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state)

これはPDB形式変換不可エントリーです。

6MTC の概要

• エントリーDOI: 10.2210/pdb6mtc/pdb
• EMDBエントリー: 9234 9235 9236 9237 9239
• 分子名称: Z-site tRNA, 60S ribosomal protein L7, 60S ribosomal protein L7a, ... (84 entities in total)
• 機能のキーワード: translation, ribosome
• 由来する生物種: Oryctolagus cuniculus (Rabbit); 詳細
• タンパク質・核酸の鎖数: 82
• 化学式量合計: 3252695.27

構造登録者

Brown, A.,Baird, M.R.,Yip, M.C.J.,Murray, J.,Shao, S. (登録日: 2018-10-19, 公開日: 2018-11-21, 最終更新日: 2024-12-25)

主引用文献

Brown, A.,Baird, M.R.,Yip, M.C.,Murray, J.,Shao, S.
Structures of translationally inactive mammalian ribosomes.
Elife, 7:-, 2018

PubMed Abstract: The cellular levels and activities of ribosomes directly regulate gene expression during numerous physiological processes. The mechanisms that globally repress translation are incompletely understood. Here, we use electron cryomicroscopy to analyze inactive ribosomes isolated from mammalian reticulocytes, the penultimate stage of red blood cell differentiation. We identify two types of ribosomes that are translationally repressed by protein interactions. The first comprises ribosomes sequestered with elongation factor 2 (eEF2) by SERPINE mRNA binding protein 1 (SERBP1) occupying the ribosomal mRNA entrance channel. The second type are translationally repressed by a novel ribosome-binding protein, interferon-related developmental regulator 2 (IFRD2), which spans the P and E sites and inserts a C-terminal helix into the mRNA exit channel to preclude translation. IFRD2 binds ribosomes with a tRNA occupying a noncanonical binding site, the 'Z site', on the ribosome. These structures provide functional insights into how ribosomal interactions may suppress translation to regulate gene expression.

PubMed: 30355441
DOI: 10.7554/eLife.40486

実験手法

ELECTRON MICROSCOPY (3.4 Å)