6MQO

Structure of HIV-1 CA G208R

6MQO の概要

• エントリーDOI: 10.2210/pdb6mqo/pdb
• 分子名称: Capsid protein, IODIDE ION (3 entities in total)
• 機能のキーワード: capsid, viral protein
• 由来する生物種: Human immunodeficiency virus 1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26115.57

構造登録者

Smaga, S.S.,Xiong, Y. (登録日: 2018-10-10, 公開日: 2019-06-19, 最終更新日: 2024-10-23)

主引用文献

Smaga, S.S.,Xu, C.,Summers, B.J.,Digianantonio, K.M.,Perilla, J.R.,Xiong, Y.
MxB Restricts HIV-1 by Targeting the Tri-hexamer Interface of the Viral Capsid.
Structure, 27:1234-, 2019

PubMed Abstract: The human antiviral protein MxB is a restriction factor that fights HIV infection. Previous experiments have demonstrated that MxB targets the HIV capsid, a protein shell that protects the viral genome. To make the conical-shaped capsid, HIV CA proteins are organized into a lattice composed of hexamer and pentamer building blocks, providing many interfaces for host proteins to recognize. Through extensive biochemical and biophysical studies and molecular dynamics simulations, we show that MxB is targeting the HIV capsid by recognizing the region created at the intersection of three CA hexamers. We are further able to map this interaction to a few CA residues, located in a negatively charged well at the interface between the three CA hexamers. This work provides detailed residue-level mapping of the targeted capsid interface and how MxB interacts. This information could inspire the development of capsid-targeting therapies for HIV.

PubMed: 31155311
DOI: 10.1016/j.str.2019.04.015

実験手法

X-RAY DIFFRACTION (3.2 Å)