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6MQF

Myotoxin II from Bothrops moojeni complexed with Acetylsalicylic acid

Summary for 6MQF
Entry DOI10.2210/pdb6mqf/pdb
Related6mqd
DescriptorBasic phospholipase A2 homolog 2, DIMETHYL SULFOXIDE, 2-(ACETYLOXY)BENZOIC ACID, ... (4 entities in total)
Functional Keywordsaspirin, acetylsalicylic acid, toxin
Biological sourceBothrops moojeni (Lance-headed viper)
Total number of polymer chains2
Total formula weight28419.13
Authors
Salvador, G.H.M.,Fontes, M.R.M. (deposition date: 2018-10-09, release date: 2019-03-20, Last modification date: 2023-10-11)
Primary citationSalvador, G.H.M.,Cardoso, F.F.,Gomes, A.A.,Cavalcante, W.L.G.,Gallacci, M.,Fontes, M.R.M.
Search for efficient inhibitors of myotoxic activity induced by ophidian phospholipase A2-like proteins using functional, structural and bioinformatics approaches.
Sci Rep, 9:510-510, 2019
Cited by
PubMed Abstract: Ophidian accidents are considered an important neglected tropical disease by the World Health Organization. Particularly in Latin America, Bothrops snakes are responsible for the majority of the snakebite envenomings that are not efficiently treated by conventional serum therapy. Thus, the search for simple and efficient inhibitors to complement this therapy is a promising research area, and a combination of functional and structural assays have been used to test candidate ligands against specific ophidian venom compounds. Herein, we tested a commercial drug (acetylsalicylic acid, ASA) and a plant compound with antiophidian properties (rosmarinic acid, RA) using myographic, crystallographic and bioinformatics experiments with a phospholipase A-like toxin, MjTX-II. MjTX-II/RA and MjTX-II/ASA crystal structures were solved at high resolution and revealed the presence of ligands bound to different regions of the toxin. However, in vitro myographic assays showed that only RA is able to prevent the myotoxic effects of MjTX-II. In agreement with functional results, molecular dynamics simulations showed that the RA molecule remains tightly bound to the toxin throughout the calculations, whereas ASA molecules tend to dissociate. This approach aids the design of effective inhibitors of PLA-like toxins and, eventually, may complement serum therapy.
PubMed: 30679550
DOI: 10.1038/s41598-018-36839-6
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.693 Å)
Structure validation

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数据于2024-11-13公开中

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