6MNR

Rhesus macaque anti-HIV V3 antibody DH753 with gp120 V3 ZAM18 peptide

6MNR の概要

• エントリーDOI: 10.2210/pdb6mnr/pdb
• 分子名称: Envelope glycoprotein, Ab DH753 heavy chain Fab fragment, Ab DH753 light chain, ... (6 entities in total)
• 機能のキーワード: hiv-1 gp120 v3 antibody, immune system
• 由来する生物種: Macaca mulatta; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 49576.97

構造登録者

Nicely, N.I. (登録日: 2018-10-02, 公開日: 2019-07-24, 最終更新日: 2024-11-06)

主引用文献

Han, Q.,Jones, J.A.,Nicely, N.I.,Reed, R.K.,Shen, X.,Mansouri, K.,Louder, M.,Trama, A.M.,Alam, S.M.,Edwards, R.J.,Bonsignori, M.,Tomaras, G.D.,Korber, B.,Montefiori, D.C.,Mascola, J.R.,Seaman, M.S.,Haynes, B.F.,Saunders, K.O.
Difficult-to-neutralize global HIV-1 isolates are neutralized by antibodies targeting open envelope conformations.
Nat Commun, 10:2898-2898, 2019

PubMed Abstract: The HIV-1 envelope (Env) is the target for neutralizing antibodies and exists on the surface of virions in open or closed conformations. Difficult-to-neutralize viruses (tier 2) express Env in a closed conformation antigenic for broadly neutralizing antibodies (bnAbs) but not for third variable region (V3) antibodies. Here we show that select V3 macaque antibodies elicited by Env vaccination can neutralize 26% of otherwise tier 2 HIV-1 isolates in standardized virus panels. The V3 antibodies only bound to Env in its open conformation. Thus, Envs on tier 2 viruses sample a state where the V3 loop is not in its closed conformation position. Envelope second variable region length, glycosylation sites and V3 amino acids were signatures of neutralization sensitivity. This study determined that open conformations of Env with V3 exposed are present on a subset of otherwise neutralization-resistant virions, therefore neutralization of tier 2 HIV-1 does not always indicate bnAb induction.

PubMed: 31263112
DOI: 10.1038/s41467-019-10899-2

実験手法

X-RAY DIFFRACTION (2.201 Å)