6MNM

6256 TCR bound to I-Ab Padi4

6MNM の概要

• エントリーDOI: 10.2210/pdb6mnm/pdb
• 分子名称: H-2 class II histocompatibility antigen, A-B alpha chain, Padi4 (92-105) peptide and MHC Class II I-Ab beta chain, 6256 TCR alpha chain, ... (4 entities in total)
• 機能のキーワード: t cell receptor, major histocompatibility complex, immune system
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 95284.81

構造登録者

Blevins, S.J.,Stadinski, B.D.,Huseby, E.S. (登録日: 2018-10-02, 公開日: 2019-07-03, 最終更新日: 2024-11-06)

主引用文献

Stadinski, B.D.,Blevins, S.J.,Spidale, N.A.,Duke, B.R.,Huseby, P.G.,Stern, L.J.,Huseby, E.S.
A temporal thymic selection switch and ligand binding kinetics constrain neonatal Foxp3+Tregcell development.
Nat.Immunol., 20:1046-1058, 2019

PubMed Abstract: The neonatal thymus generates Foxp3 regulatory T (tT) cells that are critical in controlling immune homeostasis and preventing multiorgan autoimmunity. The role of antigen specificity on neonatal tT cell selection is unresolved. Here we identify 17 self-peptides recognized by neonatal tT cells, and reveal ligand specificity patterns that include self-antigens presented in an age- and inflammation-dependent manner. Fate-mapping studies of neonatal peptidyl arginine deiminase type IV (Padi4)-specific thymocytes reveal disparate fate choices. Neonatal thymocytes expressing T cell receptors that engage IA-Padi4 with moderate dwell times within a conventional docking orientation are exported as tT cells. In contrast, Padi4-specific T cell receptors with short dwell times are expressed on CD4 T cells, while long dwell times induce negative selection. Temporally, Padi4-specific thymocytes are subject to a developmental stage-specific change in negative selection, which precludes tT cell development. Thus, a temporal switch in negative selection and ligand binding kinetics constrains the neonatal tT selection window.

PubMed: 31209405
DOI: 10.1038/s41590-019-0414-1

実験手法

X-RAY DIFFRACTION (3.1 Å)