6MJY

M. thermoresistible GuaB2 delta-CBS in complex with 6Cl-IMP

6MJY の概要

• エントリーDOI: 10.2210/pdb6mjy/pdb
• 分子名称: Inosine-5'-monophosphate dehydrogenase, 6-CHLOROPURINE RIBOSIDE, 5'-MONOPHOSPHATE (3 entities in total)
• 機能のキーワード: complex, fragment, impdh, oxidoreductase, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• 由来する生物種: Mycobacterium thermoresistibile; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40222.14

構造登録者

Ascher, D.B.,Pacitto, A.,Blundell, T.L. (登録日: 2018-09-23, 公開日: 2019-09-25, 最終更新日: 2023-10-11)

主引用文献

Trapero, A.,Pacitto, A.,Chan, D.S.,Abell, C.,Blundell, T.L.,Ascher, D.B.,Coyne, A.G.
Covalent inactivation of Mycobacterium thermoresistibile inosine-5'-monophosphate dehydrogenase (IMPDH).
Bioorg.Med.Chem.Lett., 30:126792-126792, 2020

PubMed Abstract: Inosine-5'-monophosphate dehydrogenase (IMPDH) is a rate-limiting enzyme involved in nucleotide biosynthesis. Because of its critical role in purine biosynthesis, IMPDH is a drug design target for immunosuppressive, anticancer, antiviral and antimicrobial chemotherapy. In this study, we use mass spectrometry and X-ray crystallography to show that the inhibitor 6-Cl-purine ribotide forms a covalent adduct with the Cys-341 residue of Mycobacterium thermoresistibile IMPDH.

PubMed: 31757668
DOI: 10.1016/j.bmcl.2019.126792

実験手法

X-RAY DIFFRACTION (1.56 Å)