6MJ0

Crystal structure of the complete turnip yellow mosaic virus 3'UTR

6MJ0 の概要

• エントリーDOI: 10.2210/pdb6mj0/pdb
• 分子名称: RNA (101-MER) (1 entity in total)
• 機能のキーワード: trna like-structure, trna mimicry, translation enhancer, programmed conformational changes, rna
• 由来する生物種: Turnip yellow mosaic virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66641.48

構造登録者

Hartwick, E.W.,Costantino, D.A.,MacFadden, A.,Nix, J.C.,Tian, S.,Das, R.,Kieft, J.S. (登録日: 2018-09-20, 公開日: 2019-01-09, 最終更新日: 2023-10-11)

主引用文献

Hartwick, E.W.,Costantino, D.A.,MacFadden, A.,Nix, J.C.,Tian, S.,Das, R.,Kieft, J.S.
Ribosome-induced RNA conformational changes in a viral 3'-UTR sense and regulate translation levels.
Nat Commun, 9:5074-5074, 2018

PubMed Abstract: Structured RNA elements, programmed RNA conformational changes, and interactions between different RNA domains underlie many modes of regulating gene expression, mandating studies to understand the foundational principles that govern these phenomena. Exploring the structured 3' untranslated region (UTR) of a viral RNA, we discovered that different contexts of the 3'-UTR confer different abilities to enhance translation of an associated open reading frame. In one context, ribosome-induced conformational changes in a 'sensor' RNA domain affect a separate RNA 'functional' domain, altering translation efficiency. The structure of the entire 3'-UTR reveals that structurally distinct domains use a spine of continuously stacked bases and a strut-like linker to create a conduit for communication within the higher-order architecture. Thus, this 3'-UTR RNA illustrates how RNA can use programmed conformational changes to sense the translation status of an upstream open reading frame, then create a tuned functional response by communicating that information to other RNA elements.

PubMed: 30498211
DOI: 10.1038/s41467-018-07542-x

実験手法

X-RAY DIFFRACTION (3.1 Å)