6MIG

Crystal structure of host-guest complex with PB hachimoji DNA

6MIG の概要

• エントリーDOI: 10.2210/pdb6mig/pdb
• 分子名称: Gag-Pol polyprotein, DNA (5'-D(*CP*TP*TP*AP*TP*(1WA)P*(1WA)P*(DS))-3'), DNA (5'-D(P*(DB)P*(1W5)P*(1W5)P*AP*TP*AP*AP*G)-3'), ... (4 entities in total)
• 機能のキーワード: host-guest complex, hachimoji dna, n-terminal fragment of mmlv rt, dna, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Moloney murine leukemia virus (isolate Shinnick) (MoMLV); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 34260.05

構造登録者

Georgiadis, M.M. (登録日: 2018-09-19, 公開日: 2019-02-27, 最終更新日: 2024-03-13)

主引用文献

Hoshika, S.,Leal, N.A.,Kim, M.J.,Kim, M.S.,Karalkar, N.B.,Kim, H.J.,Bates, A.M.,Watkins Jr., N.E.,SantaLucia, H.A.,Meyer, A.J.,DasGupta, S.,Piccirilli, J.A.,Ellington, A.D.,SantaLucia Jr., J.,Georgiadis, M.M.,Benner, S.A.
Hachimoji DNA and RNA: A genetic system with eight building blocks.
Science, 363:884-887, 2019

PubMed Abstract: We report DNA- and RNA-like systems built from eight nucleotide "letters" (hence the name "hachimoji") that form four orthogonal pairs. These synthetic systems meet the structural requirements needed to support Darwinian evolution, including a polyelectrolyte backbone, predictable thermodynamic stability, and stereoregular building blocks that fit a Schrödinger aperiodic crystal. Measured thermodynamic parameters predict the stability of hachimoji duplexes, allowing hachimoji DNA to increase the information density of natural terran DNA. Three crystal structures show that the synthetic building blocks do not perturb the aperiodic crystal seen in the DNA double helix. Hachimoji DNA was then transcribed to give hachimoji RNA in the form of a functioning fluorescent hachimoji aptamer. These results expand the scope of molecular structures that might support life, including life throughout the cosmos.

PubMed: 30792304
DOI: 10.1126/science.aat0971

実験手法

X-RAY DIFFRACTION (1.7 Å)