6MG9

Human Obscurin Ig57 Domain

Summary for 6MG9

• Entry DOI: 10.2210/pdb6mg9/pdb
• NMR Information: BMRB: 30514
• Descriptor: Obscurin (1 entity in total)
• Functional Keywords: obscurin, ig domain, wlc, structural protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 11112.37

Authors

Wright, N.T.,Whitley, J.A. (deposition date: 2018-09-13, release date: 2019-02-06, Last modification date: 2024-05-01)

Primary citation

Whitley, J.A.,Ex-Willey, A.M.,Marzolf, D.R.,Ackermann, M.A.,Tongen, A.L.,Kokhan, O.,Wright, N.T.
Obscurin is a semi-flexible molecule in solution.
Protein Sci., 28:717-726, 2019

PubMed Abstract: Obscurin, a giant modular cytoskeletal protein, is comprised mostly of tandem immunoglobulin-like (Ig-like) domains. This architecture allows obscurin to connect distal targets within the cell. The linkers connecting the Ig domains are usually short (3-4 residues). The physical effect arising from these short linkers is not known; such linkers may lead to a stiff elongated molecule or, conversely, may lead to a more compact and dynamic structure. In an effort to better understand how linkers affect obscurin flexibility, and to better understand the physical underpinnings of this flexibility, here we study the structure and dynamics of four representative sets of dual obscurin Ig domains using experimental and computational techniques. We find in all cases tested that tandem obscurin Ig domains interact at the poles of each domain and tend to stay relatively extended in solution. NMR, SAXS, and MD simulations reveal that while tandem domains are elongated, they also bend and flex significantly. By applying this behavior to a simplified model, it becomes apparent obscurin can link targets more than 200 nm away. However, as targets get further apart, obscurin begins acting as a spring and requires progressively more energy to further elongate.

PubMed: 30666746
DOI: 10.1002/pro.3578

Experimental method

SOLUTION NMR