6M79

Cryo-EM structure of Arabidopsis CRY under blue light-mediated activation

Summary for 6M79

• Entry DOI: 10.2210/pdb6m79/pdb
• EMDB information: 30128
• Descriptor: Cryptochrome-2, ADENOSINE MONOPHOSPHATE, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total)
• Functional Keywords: blue-light activated, arabidopsis thaliana, cry2 tetramer, transcription
• Biological source: Arabidopsis thaliana (Mouse-ear cress)
• Total number of polymer chains: 4
• Total formula weight: 282850.80

Authors

Ma, L.,Guan, Z.Y.,Yin, P. (deposition date: 2020-03-18, release date: 2020-10-14, Last modification date: 2025-09-17)

Primary citation

Ma, L.,Guan, Z.,Wang, Q.,Yan, X.,Wang, J.,Wang, Z.,Cao, J.,Zhang, D.,Gong, X.,Yin, P.
Structural insights into the photoactivation of Arabidopsis CRY2.
Nat.Plants, 6:1432-1438, 2020

PubMed Abstract: The blue-light receptor cryptochrome (CRY) in plants undergoes oligomerization to transduce blue-light signals after irradiation, but the corresponding molecular mechanism remains poorly understood. Here, we report the cryogenic electron microscopy structure of a blue-light-activated CRY2 tetramer at a resolution of 3.1 Å, which shows how the CRY2 tetramer assembles. Our study provides insights into blue-light-mediated activation of CRY2 and a theoretical basis for developing regulators of CRYs for optogenetic manipulation.

PubMed: 33199893
DOI: 10.1038/s41477-020-00800-1

Experimental method

ELECTRON MICROSCOPY (3.1 Å)