6M3W

Post-fusion structure of SARS-CoV spike glycoprotein

6M3W の概要

• エントリーDOI: 10.2210/pdb6m3w/pdb
• EMDBエントリー: 30072
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: spike, post-fusion, sars-cov, coronavirus, glycoprotein, trimer, viral protein
• 由来する生物種: Human SARS coronavirus (SARS-CoV)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 167585.36

構造登録者

Fan, X.,Cao, D.,Zhang, X. (登録日: 2020-03-04, 公開日: 2020-06-03, 最終更新日: 2025-07-02)

主引用文献

Fan, X.,Cao, D.,Kong, L.,Zhang, X.
Cryo-EM analysis of the post-fusion structure of the SARS-CoV spike glycoprotein.
Nat Commun, 11:3618-3618, 2020

PubMed Abstract: Global emergencies caused by the severe acute respiratory syndrome coronavirus (SARS-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and SARS-CoV-2 significantly endanger human health. The spike (S) glycoprotein is the key antigen and its conserved S2 subunit contributes to viral entry by mediating host-viral membrane fusion. However, structural information of the post-fusion S2 from these highly pathogenic human-infecting coronaviruses is still lacking. We used single-particle cryo-electron microscopy to show that the post-fusion SARS-CoV S2 forms a further rotated HR1-HR2 six-helix bundle and a tightly bound linker region upstream of the HR2 motif. The structures of pre- and post-fusion SARS-CoV S glycoprotein dramatically differ, resembling that of the Mouse hepatitis virus (MHV) and other class I viral fusion proteins. This structure suggests potential targets for the development of vaccines and therapies against a wide range of SARS-like coronaviruses.

PubMed: 32681106
DOI: 10.1038/s41467-020-17371-6

実験手法

ELECTRON MICROSCOPY (3.9 Å)