6M2O

Double mutant(H333A/I334A) crystal structure of benzoate coenzyme A ligase

6M2O の概要

• エントリーDOI: 10.2210/pdb6m2o/pdb
• 分子名称: Benzoate-coenzyme A ligase (2 entities in total)
• 機能のキーワード: ligase
• 由来する生物種: Rhodopseudomonas palustris
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 113366.20

構造登録者

Li, T.L.,Adhikari, K. (登録日: 2020-02-28, 公開日: 2021-01-13, 最終更新日: 2023-11-29)

主引用文献

Adhikari, K.,Lo, I.W.,Chen, C.L.,Wang, Y.L.,Lin, K.H.,Zadeh, S.M.,Rattinam, R.,Li, Y.S.,Wu, C.J.,Li, T.L.
Chemoenzymatic Synthesis and Biological Evaluation for Bioactive Molecules Derived from Bacterial Benzoyl Coenzyme A Ligase and Plant Type III Polyketide Synthase.
Biomolecules, 10:-, 2020

PubMed Abstract: Plant type III polyketide synthases produce diverse bioactive molecules with a great medicinal significance to human diseases. Here, we demonstrated versatility of a stilbene synthase (STS) from , which can accept various non-physiological substrates to form unnatural polyketide products. Three enzymes (4-coumarate CoA ligase, malonyl-CoA synthetase and engineered benzoate CoA ligase) along with synthetic chemistry was practiced to synthesize starter and extender substrates for STS. Of these, the crystal structures of benzoate CoA ligase (BadA) from in an form or in complex with a 2-chloro-1,3-thiazole-5-carboxyl-AMP or 2-methylthiazole-5-carboxyl-AMP intermediate were determined at resolutions of 1.57 Å, 1.7 Å, and 2.13 Å, respectively, which reinforces its capacity in production of unusual CoA starters. STS exhibits broad substrate promiscuity effectively affording structurally diverse polyketide products. Seven novel products showed desired cytotoxicity against a panel of cancer cell lines (A549, HCT116, Cal27). With the treatment of two selected compounds, the cancer cells underwent cell apoptosis in a dose-dependent manner. The precursor-directed biosynthesis alongside structure-guided enzyme engineering greatly expands the pharmaceutical repertoire of lead compounds with promising/enhanced biological activities.

PubMed: 32397467
DOI: 10.3390/biom10050738

実験手法

X-RAY DIFFRACTION (1.57 Å)