6M2M

A role for histone chaperone OsChz1 in histone recognition and deposition

6M2M の概要

• エントリーDOI: 10.2210/pdb6m2m/pdb
• 分子名称: Probable histone H2A.3, Histone H2B.1, Expressed protein, ... (5 entities in total)
• 機能のキーワード: complex, chaperone
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 161121.70

構造登録者

Luo, Q.,Wang, B. (登録日: 2020-02-28, 公開日: 2020-10-21, 最終更新日: 2024-03-27)

主引用文献

Du, K.,Luo, Q.,Yin, L.,Wu, J.,Liu, Y.,Gan, J.,Dong, A.,Shen, W.H.
OsChz1 acts as a histone chaperone in modulating chromatin organization and genome function in rice.
Nat Commun, 11:5717-5717, 2020

PubMed Abstract: While the yeast Chz1 acts as a specific histone-chaperone for H2A.Z, functions of CHZ-domain proteins in multicellular eukaryotes remain obscure. Here, we report on the functional characterization of OsChz1, a sole CHZ-domain protein identified in rice. OsChz1 interacts with both the canonical H2A-H2B dimer and the variant H2A.Z-H2B dimer. Within crystal structure the C-terminal region of OsChz1 binds H2A-H2B via an acidic region, pointing to a previously unknown recognition mechanism. Knockout of OsChz1 leads to multiple plant developmental defects. At genome-wide level, loss of OsChz1 causes mis-regulations of thousands of genes and broad alterations of nucleosome occupancy as well as reductions of H2A.Z-enrichment. While OsChz1 associates with chromatin regions enriched of repressive histone marks (H3K27me3 and H3K4me2), its loss does not affect the genome landscape of DNA methylation. Taken together, it is emerging that OsChz1 functions as an important H2A/H2A.Z-H2B chaperone in dynamic regulation of chromatin for higher eukaryote development.

PubMed: 33177521
DOI: 10.1038/s41467-020-19586-z

実験手法

X-RAY DIFFRACTION (2.85 Å)