6LY4

The crystal structure of the BM3 mutant LG-23 in complex with testosterone

Summary for 6LY4

Entry DOI	10.2210/pdb6ly4/pdb
Descriptor	Bifunctional cytochrome P450/NADPH--P450 reductase, TESTOSTERONE, IMIDAZOLE, ... (6 entities in total)
Functional Keywords	p450-bm3 mutant, regio- and stereoselectivity, hydroxylation, testosterone, oxidoreductase
Biological source	Bacillus megaterium (strain ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / VKM B-512)
Total number of polymer chains	1
Total formula weight	56800.28
Authors	Peng, Y.,Chen, J.,Zhou, J.,Li, A.,ReetZ, M.T. (deposition date: 2020-02-13, release date: 2020-04-29, Last modification date: 2023-11-29)
Primary citation	Li, A.,Acevedo-Rocha, C.G.,D'Amore, L.,Chen, J.,Peng, Y.,Garcia-Borras, M.,Gao, C.,Zhu, J.,Rickerby, H.,Osuna, S.,Zhou, J.,Reetz, M.T. Regio- and Stereoselective Steroid Hydroxylation at C7 by Cytochrome P450 Monooxygenase Mutants. Angew.Chem.Int.Ed.Engl., 59:12499-12505, 2020 Cited by PubMed: 32243054 DOI: 10.1002/anie.202003139 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.68 Å)

Structure validation