6LU8

Cryo-EM structure of a human pre-60S ribosomal subunit - state A

これはPDB形式変換不可エントリーです。

6LU8 の概要

• エントリーDOI: 10.2210/pdb6lu8/pdb
• EMDBエントリー: 0978
• 分子名称: Nucleolar GTP-binding protein 2, 60S ribosomal protein L10a, 60S ribosomal protein L3, ... (52 entities in total)
• 機能のキーワード: 60s, pre-60s, pre-ribosome, human 60s, human pre-ribosome, nmd3, human nmd3, ribosome
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 50
• 化学式量合計: 2845828.24

構造登録者

Liang, X.,Zuo, M.,Zhang, Y.,Li, N.,Ma, C.,Dong, M.,Gao, N. (登録日: 2020-01-26, 公開日: 2020-08-26, 最終更新日: 2025-09-17)

主引用文献

Liang, X.,Zuo, M.Q.,Zhang, Y.,Li, N.,Ma, C.,Dong, M.Q.,Gao, N.
Structural snapshots of human pre-60S ribosomal particles before and after nuclear export.
Nat Commun, 11:3542-3542, 2020

PubMed Abstract: Ribosome biogenesis is an elaborate and energetically expensive program that involve two hundred protein factors in eukaryotes. Nuclear export of pre-ribosomal particles is one central step which also serves as an internal structural checkpoint to ensure the proper completion of nuclear assembly events. Here we present four structures of human pre-60S particles isolated through a nuclear export factor NMD3, representing assembly stages immediately before and after nuclear export. These structures reveal locations of a dozen of human factors, including an uncharacterized factor TMA16 localized between the 5S RNA and the P0 stalk. Comparison of these structures shows a progressive maturation for the functional regions, such as peptidyl transferase centre and peptide exit tunnel, and illustrate a sequence of factor-assisted rRNA maturation events. These data facilitate our understanding of the global conservation of ribosome assembly in eukaryotes and species-specific features of human assembly factors.

PubMed: 32669547
DOI: 10.1038/s41467-020-17237-x

実験手法

ELECTRON MICROSCOPY (3.13 Å)