6LTR

Crystal structure of Cas12i2 ternary complex with single Mg2+ bound in catalytic pocket

Summary for 6LTR

• Entry DOI: 10.2210/pdb6ltr/pdb
• Descriptor: Cas12i2, RNA (56-mer), DNA (5'-D(*GP*CP*CP*GP*CP*TP*TP*TP*CP*TP*TP*T)-3'), ... (8 entities in total)
• Functional Keywords: crispr-cas, cas12i2, cas12i2 ternary complex, hydrolase, hydrolase-rna-dna complex, hydrolase/rna/dna
• Biological source: unidentified; More
• Total number of polymer chains: 5
• Total formula weight: 155950.18

Authors

Huang, X.,Sun, W.,Cheng, Z.,Chen, M.,Li, X.,Wang, J.,Sheng, G.,Gong, W.,Wang, Y. (deposition date: 2020-01-23, release date: 2020-10-28, Last modification date: 2024-03-27)

• Primary citation: Huang, X.,Sun, W.,Cheng, Z.,Chen, M.,Li, X.,Wang, J.,Sheng, G.,Gong, W.,Wang, Y.; Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2.; Nat Commun, 11:5241-5241, 2020; PubMed: 33067443; DOI: 10.1038/s41467-020-19072-6

Experimental method

X-RAY DIFFRACTION (2.51 Å)