6LQT

Cryo-EM structure of 90S small subunit preribosomes in transition states (State E)

これはPDB形式変換不可エントリーです。

6LQT の概要

• エントリーDOI: 10.2210/pdb6lqt/pdb
• 関連するPDBエントリー: 6LQP 6LQQ 6LQR 6LQS 6LQU 6LQV
• EMDBエントリー: 0949 0950 0951 0952 0953 0954 0955
• 分子名称: U3 snoRNA, 40S ribosomal protein S9-A, 40S ribosomal protein S11-A, ... (59 entities in total)
• 機能のキーワード: ribosome assembly, 90s to pre-40s transition, cryo-em, dhr1, ribosome
• 由来する生物種: Saccharomyces cerevisiae S288C (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 58
• 化学式量合計: 4119341.19

構造登録者

Du, Y.,Ye, K. (登録日: 2020-01-14, 公開日: 2020-09-16, 最終更新日: 2024-03-27)

主引用文献

Du, Y.,An, W.,Zhu, X.,Sun, Q.,Qi, J.,Ye, K.
Cryo-EM structure of 90 S small ribosomal subunit precursors in transition states.
Science, 369:1477-1481, 2020

PubMed Abstract: The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by determining cryo-electron microscopy structures of intermediates in the path from the 90 to the pre-40 The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5' external transcribed spacer (5' ETS) is degraded within 90, driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90 through helicase Mtr4 and is primed to digest the 3' end of the 5' ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5' ETS degradation in 90 progression.

PubMed: 32943522
DOI: 10.1126/science.aba9690

実験手法

ELECTRON MICROSCOPY (4.9 Å)